Yogyakarta, August 24, 2024 – The Plant Systematics Laboratory organized a training on Plant DNA Barcoding to introduce and enhance molecular data processing competencies. The activity included DNA extraction, PCR techniques for DNA amplification, agarose gel electrophoresis, DNA barcoding, and DNA sequence analysis for systematic research—the event attended by 15 participants from the Faculty of Biology and the Graduate School of UGM.
The event opened by the Dean of the Faculty of Biology, Prof. Dr. Budi Setiadi Daryono, M.Agr.Sc. He stated, “The diversity of living organisms is vast, and the genetic diversity is still not widely explored. This training was an excellent agenda to expand knowledge on genetic diversity.” Following this, the Head of the Plant Systematics Laboratory at the Faculty of Biology UGM, Prof. Dr. Ratna Susandarini, M.Sc., delivered her remarks, expressing appreciation for the participant’s enthusiasm in joining the training sessions.
The day one training involved lectures and practical sessions delivered by Dr. Istiana Prihatini, M.Si. from the Center for Applied Botanical Research, National Research and Innovation Agency (BRIN), on plant DNA extraction using the conventional CTAB method and DNA extraction kit. Dry and fresh leaf samples used for the DNA extraction practical session. All participants successfully isolated DNA from their samples, which will used for DNA amplification on the second day training.
The day two training involved the preparation of PCR (Polymerase Chain Reaction) using the DNA samples extracted the day before. This stage aimed to amplify specific DNA segments for further analysis. Subsequently, participants were given a lecture on the basic principles of PCR and sequencing by Prof. Dr. Ratna Susandarini, M.Sc., highlighting the essential steps in genetic analysis. Participants were also equipped with knowledge about DNA Barcoding, an essential technique in plant systematics research, presented by Abdul Razaq Chasani, M.Si, Ph.D., a lecturer at the Plant Systematics Laboratory. After the presentation, the analysis continued with electrophoresis.
One of the participants, Nabila from the Faculty of Biology UGM, appreciated the training activities. “The training was fascinating and enjoyable. The speakers and facilitators delivered theory and practical sessions excellently, clearly, and structured. The understanding of the DNA Barcoding process from start to finish was explained in detail, including data analysis.”
The Plant DNA Barcoding Training reflects the contribution of the Faculty of Biology UGM, particularly the Plant Systematics Laboratory, in supporting the Sustainable Development Goals (SDGs) by providing high-quality training to enhance education (SDG No. 4). Furthermore, the DNA Barcoding training materials can improve participants’ skills, which can later provide positive benefits for the conservation of terrestrial ecosystems in line with SDG No. 15.
The event was officially closed by the Head of the Plant Systematics Laboratory, followed by a group photo session. This two-day training emphasized the importance of strengthening the capacity and capabilities of the Plant Systematics Laboratory in molecular activities, particularly DNA Barcoding.
