BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF A NEW ISOLATE OF CUCUMBER GREEN MOTTLE MOSAIC VIRUS (CGMMV) IN INDONESIA

BIOLOGICAL AND MOLECULAR CHARACTERIZATION OF A NEW ISOLATE OF

CUCUMBER GREEN MOTTLE MOSAIC VIRUS (CGMMV) IN INDONESIA

 

Budi Setiadi Daryono¹, Tri Joko², Alin Liana³, Utari Saraswati^1
1 Genetics Laboratory, Faculty of Biology, Gadjah Mada University, Yogyakarta 55281 Indonesia (Corresponding e-mail: bs_daryono@mail.ugm.ac.id)
² Laboratory of Agricultural Biotechnology, Faculty of Agriculture, Gadjah Mada University, Yogyakarta 55281 Indonesia
³ Faculty of Mathematics and Natural Sciences STKIP Pembangunan Indonesia Makasar, Makasar Indonesia

Cucurbits grow throughout the Java island of Indonesia as dry season crops. However, the cucurbits ultivation has been faced with virus infections that reduce their production. Cucumber green mottle mosaic virus (CGMMV), which a member of the Genus Tobamovirus, has been reported infecting cucurbits in Indonesia. The most common symptoms of infected plants are leaf mosaic and mottling, growth stunting and malformation of leaf and fruit. The purpose of this research were to find out the occurrence of CGMMV infecting cucurbits in Daerah Istimewa Yogyakarta (DIY) and to identify the biological and molecular haracterization. Survey and samples collection were conducted in several fields in Sleman and Kulon progo, DIY during March – June 2009. Serological analysis confirmed that two angled loofah (Luffa acutangula L.) were positively infected by a virus related to Cucumber green mottle mosaic virus (CGMMV), and called CGMMV-ILa isolate. The reaction of indicator plants to CGMMV-ILa were the necrotic local lesion on Chenopodium amaranticolor, Chenopodium quinoa, and Momordica charantia. The mosaic symptoms on Citrullus lanatus, Cucumis sativus, Luffa acutangula, Nicotiana tabacum var. Virginia, and the chlorotic symptoms on Cucumis melo, but the CGMMV-ILa did not infect Lycopersicon esculentum. The coat protein (CP) of purified CGMMV-ILa detected with molecular weight of about 16 kDa by SDSPAGE analysis. Viral and complementary CGMMV-specific primer sets were designed for spanning the genome using previously reported CGMMV sequence. About 500bp of Coat protein (CP) gene of CGMMVILa was amplified by RT-PCR. The nucleotide sequence of CP gene of CGMMV- ILa shared 81% identities with several isolate of CGMMV. Based on these result, we identified CGMMV- ILa as a CGMMV isolate of Luffa acutangula, a member of Tobamovirus.