A research team from the Faculty of Biology, Universitas Gadjah Mada (UGM), has investigated metabolite profiles in the leaves, callus tissues, and culture medium exudates of red okra (Abelmoschus esculentus (L.) Moench var. ‘Zahira IPB’). The study was led by Woro Anindito Sri Tunjung, S.Si., M.Sc., Ph.D., together with Achmad Nadhmi Arfa, Elisabeth Kurniawati, and Ferdinan Florian. Conducted from August 2025 to May 2026, the research was funded by the 2026 Lecturer–Student Collaborative Research Grant. Experimental work was carried out at Biodiversity Research Station II, the Biotechnology Laboratory and Biochemistry Laboratory of the Faculty of Biology UGM, and Corpora Science.
Red okra was selected because it is recognized as a functional food rich in bioactive compounds, including quercetin, mucilage polysaccharides, and amino acids associated with health-promoting properties. In addition, its anthocyanin content may contribute to stronger antioxidant activity than that of conventional green okra. Despite its potential, comprehensive information on the metabolite profile of the Zahira IPB red okra variety remains limited. Moreover, metabolite composition in conventionally cultivated plants can vary in response to environmental factors such as temperature, light intensity, and nutrient availability. To minimize these sources of variation, the researchers employed in vitro tissue culture, which provides a more controlled growth environment. Using an untargeted metabolomics approach, the study characterized the detected compounds in leaves, callus tissues, and culture medium exudates to compare metabolite distribution among the different sample types.
The research began with the cultivation of okra plants to obtain explants, followed by callus induction through tissue culture, metabolite extraction, and metabolite profiling. Chemical analysis was performed using Liquid Chromatography–High Resolution Mass Spectrometry (LC–HRMS) with an untargeted metabolomics workflow. Unlike targeted analyses that focus on predefined compounds, this approach enables the comprehensive detection of a broad range of metabolites, providing an overview of the metabolite profiles present in each sample.
The findings expand current knowledge of the metabolite profile of the Zahira IPB red okra variety and provide a valuable resource for future studies in plant biotechnology and metabolomics. Beyond their scientific contribution, the results also support the Sustainable Development Goals (SDGs), particularly SDG 3 (Good Health and Well-Being) by generating scientific evidence that can facilitate the development of plant-derived pharmaceutical ingredients and health products, and SDG 12 (Responsible Consumption and Production) by demonstrating how biotechnology can promote the sustainable use of biological resources.




































































