In recent years, sequencing technology has become one of the innovative solutions for analyzing genomes. Today, sequencing technology is able to answer various challenges in research related to the biology of humans, plants, animals, pathogens, and the environment. For this reason, in an effort to develop knowledge, innovation and skills in research, on January 28-29 2023 one of the UGM Faculty of Biology lecturers, Dr. dr. Rr. Upiek Ngesti Wibawaning Astuti, DAP&E, M.Biomed attended a seminar which was held in a hybrid manner, followed by a workshop and a Lab Tour organized by PT Genomic Solidaritas Indonesia (GSI Lab) and took place at the Indonesia Institute for Life Science (iL3) in Jakarta.
The topic of the Webinar was “Multiple PCR-Based Nanopore Sequencing and Epidemiological Research” delivered by Prof. Won-Keun Kim, Ph.D, from the Department of Microbiology College of Medicine Haliyim University. He conveyed the need and importance of research on viruses, related to “Recent Emerging Virus Outbreaks and Infectious Disease”. Genomic surveillance and epidemiological studies continue to be carried out to identify sources and origins of infection, track the spread of pathogens, understand the evolutionary process of emerging viruses, so as to be able to analyze and predict the occurrence of endemics, especially those caused by viruses. On this occasion, various researches related to rodent-borne Paramyxovirus in various countries were presented which infect vertebrates from fish to mammals.
At the workshop, Oxford Nanopore Technologies (ONT) was introduced as a sequencing technology that can be used in a variety of genomic applications, such as genome mapping, mutation identification, as well as analysis of genomic variability, from which accurate, real-time genome data can be obtained with processing time. which is faster. Dr.rer.nat. Aril Aditya Parikesit, S.Si., M.Si, presented the use of this technology for pathogen identification, antibiotic resistance, and other studies. He also explained why sequencing is needed and how we plan and carry out nanopore sequencing. For this reason, it is necessary to prepare 4 main things in research with this technology, namely “background intention, sample type, methods, and results analysis”. On this occasion, the 40 workshop participants were divided into 5 groups and carried out sequencing practices. This activity ended with a GSI Lab Lab-Tour at PT Genomic, on Sultan Agung Road, Guntur, Setiabudi Jakarta, which begins with an explanation of various laboratories with their facilities, functions and level of security, and continues with visits to various laboratory rooms along with the latest equipment for sequencing and other services.
























On this very morning (22/02/2023), the day started with a clear sky and the temperatures in Yamagata were still cold (-5℃). We began our morning at 8 am as usual, heading to Yamagata University and arriving around 9 am ready to have breakfast brought by Prof. Yokoyama. After another delicious breakfast we all head to the laboratory to prepare our task for the day. 
After all the groups presented their worked and answer the questions, Prof. Jun Yokoyama handed us the certificates of achievement for successfully completing the Sakura Science program 2023 to all participants. The certificates were signed by the President of the Japan Science and Technology Agency, Dr. Hashimoto Kazuhito. Prof Yokoyama read the certificate to each of the participants of the Sakura Science program 2023 in that awarding ceremony. 














sequencing reaction. 









In the afternoon, between the continuous experiments, we were given warm and savory chicken curry with naan on the side. After we finished our DNA isolation, we stored them away to be put through PCR and excitedly waited for the results tomorrow, hoping that our efforts will pay off. At 17.30, we once again had dinner at the Indian restaurant from the night before, where we ate curry that we chose beforehand, all the while chatting with the lab assistants who joined us that evening. With the evening ending on a great note, we parted ways once again to roam the city and eventually call it a night. (Niki&Reiz)







































